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AS/NZS 4276.17.1:2000
$87.70
Water microbiology, Method 17.1: Spores of sulfite-reducing anaerobes (clostridia) including Clostridium perfringens — Membrane filtration method
This Standard sets out a method for enumerating spores of sulfite-reducing clostridia and C.perfringens in water, using membrane filtration, with a confirmation procedure for identifying C.perfringen.
Table of contents
Header
About this publication
Preface
1 Scope
2 Referenced documents
3 Principle
4 Media, reagents, and reference cultures
4.1 Culture media (see AS 4276.2 and Appendix B).
4.1.1 Tryptose sulfite cycloserine agar (without egg yolk) (TSC).
4.1.2 Lactose gelatin medium (LG).
4.1.3 Nutrient agar (NA).
4.1.4 Nitrate motility medium (NM).
4.2 Nitrate detection reagents (see Appendix B)
4.2.1 Solution A
4.2.2 Solution B
4.3 Reference culture
4.3.1 Positive culture
4.3.2 Negative culture
4.3.3 Use of reference culture
5 Apparatus
5.1 Filtration equipment and 0.45μm membranes
5.2 Anaerobic incubator/jar
5.3 Incubator or water bath
6 Test procedures
6.1 Membrane filtration method
7 Confirmation of C.perfringens
8 Report
9 Bibliography
Appendix A
Appendix B
B1 Scope
B2 Tryptose sulfite cycloserine agar
B3 Lactose gelatin medium
B4 Nitrate motility medium
B5 Nitrite detection reagents
B5.1 Solution A, Sulfanilic acid reagent
B5.2 Solution B, N-(1-naphthyl)ethylenediamine reagent
Cited references in this standard
Please select a variation to view its description.
| Published | 20/11/2000 |
|---|---|
| Pages | 7 |
Please select a variation to view its pdf.
