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AS ISO 20776.1:2024

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AS ISO 20776.1:2024

$149.01

Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices, Part 1: Broth micro-dilution reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases

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AS ISO 20776.1:2024 identically adopts ISO 20776 1:2019, which describes one reference method, broth micro-dilution, for determination of MICs. The MIC can be a guide for the clinician, and reflects the activity of the drug under the described test conditions, by taking into account other factors, such as drug pharmacology, pharmacokinetics, or bacterial resistance mechanisms

Table of contents
Header
About this publication
Preface
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Test procedures
4.1 General
4.2 Medium
4.3 Antimicrobial agents
4.3.1 General
4.3.2 Preparation of stock solutions
4.3.3 Preparation of working solutions
4.3.4 Preparation of micro-dilution trays
4.3.5 Storage of micro-dilution trays
4.4 Preparation of inoculum
4.4.1 General
4.4.2 Broth culture method
4.4.3 Direct colony suspension method
4.5 Inoculation of micro-dilution trays
4.6 Incubation of micro-dilution trays
4.7 Reading results
4.8 Special test situations where the MIC result might give unreliable results
5 Quality control
Annex A
A.1 General
A.2 Testing of non-fastidious organisms in Mueller-Hinton broth
A.2.1 General
A.2.2 Cation adjustment and content
A.2.3 Medium adjustments to reference Mueller-Hinton broth micro-dilution medium for testing certain bacterial species and other microbial agents
A.2.3.1 Streptococcus species
A.2.3.2 Sulphonamides and trimethoprim
A.2.3.3 Tigecycline
A.2.3.4 Lipoglycopeptides (dalbavancin, televancin and oritivancin)
A.2.3.5 Cefiderocol
A.2.3.6 Oxacillin testing for Staphylococci
A.2.3.7 Daptomycin
A.2.3.8 Carbapenems
A.2.3.9 Glycopeptides
A.2.3.10 Polymyxins (Colistin)
A.2.4 Supplementary medium issues for which international standardization is lacking
A.2.4.1 General
Annex B
Annex C
Annex D
D.1 General
D.2 Special test situations
D.2.1 Fastidious aerobic and facultative anaerobic bacterial species (e.g. Haemophilus)
D.2.2 Mecillinam
D.2.3 Fosfomycin
Bibliography

Cited references in this standard
Content history
DR AS ISO 20776.1:2024

Please select a variation to view its description.

Published

06/12/2024
Pages

19

Please select a variation to view its pdf.

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AS ISO 20776.1:2024
$149.01