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AS 5013.8.2:2019
$163.91
Food microbiology, Method 8.2: Microbiology of the food chain — Horizontal method for the detection and enumeration of Enterobacteriaceae — Colony-count technique (ISO 21528-2:2017, MOD)
This Standard is an adoption with national modifications, which specifies a method for the enumeration of Enterobacteriaceae.
Table of contents
Header
About this publication
Preface
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Preparation of initial suspension and decimal dilutions
4.2 Isolation and selection for confirmation
4.3 Confirmation
4.4 Calculation
5 Diluent, culture media and reagent
6 Equipment and consumables
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
6.9
6.10
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 General
9.2 Test portion, initial suspension and dilutions
9.3 Inoculation and incubation
9.3.1
9.3.2
9.3.3
9.3.4
9.4 Counting and selection of colonies for confirmation
9.5 Subculturing selected colonies
9.6 Biochemical confirmation tests
9.6.1 Oxidase reaction
9.6.2 Fermentation test
9.6.3 Interpretation of biochemical tests
10 Expression of results
11 Performance characteristics of the method
11.1 Interlaboratory study
11.2 Repeatability limit
11.3 Reproducibility limit
12 Test report
13 Quality assurance
Annex A
A.1 Diluent
A.2 Violet red bile glucose (VRBG) agar
A.2.1 Composition
A.2.2 Preparation
A.2.3 Performance testing for the quality assurance of the culture medium
A.3 Non-selective agar medium
A.3.1 General
A.3.2 Composition nutrient agar
A.3.3 Preparation
A.3.4 Preparation of agar plates
A.3.5 Performance testing for the quality assurance of the culture medium
A.4 Glucose OF medium
A.4.1 Composition
A.4.2 Preparation
A.4.3 Performance testing for the quality assurance
A.5 Oxidase reagent
A.5.1 Composition
A.5.2 Preparation
A.5.3 Performance testing for the quality assurance
A.6 Sterile mineral oil
Annex B
Bibliography
Appendix ZZ
ZZ1 General
ZZ2 Variations
Cited references in this standard
Please select a variation to view its description.
| Published | 18/01/2019 |
|---|---|
| Pages | 19 |
Please select a variation to view its pdf.
