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AS 5013.8.1:2018
$149.01
Food microbiology, Method 8.1: Microbiology of the food chain — Horizontal method for the detection and enumeration of Enterobacteriaceae — Detection of Enterobacteriaceae (ISO 21528-1:2017, MOD)
Adopts ISO 21528-1:2017, which specifies a method, with enrichment, for the detection of Enterobacteriaceae.
Table of contents
Header
About this publication
Preface
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Enrichment in non-selective medium
4.2 Isolation and selection for confirmation
4.3 Confirmation
5 Diluent, culture media and reagent
6 Equipment and consumables
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
6.9
6.10
6.11
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 General
9.2 Test portion and initial suspension
9.3 Enrichment
9.4 Isolation and selection for confirmation
9.4.1 Isolation
9.4.2 Selection of colonies for confirmation
9.5 Subculturing selected colonies
9.6 Biochemical confirmation tests
9.6.1 Oxidase reaction
9.6.2 Fermentation test
10 Expression of results
11 Precision
11.1 Interlaboratory study
11.2 Sensitivity
11.3 Specificity
12 Test report
13 Quality assurance
Annex A
A.1 General
A.2 Principle
A.2.1 Enrichment
A.2.2 Isolation and selection for confirmation
A.2.3 Confirmation
A.2.4 Calculation
A.3 Procedure (see Figure A.1)
A.3.1 Test portion, initial suspension and further dilutions
A.3.2 Enrichment
A.3.3 Isolation, subculturing and confirmation
A.4 Expression of results
A.5 Precision
Annex B
B.1 Enrichment medium and diluent: buffered peptone water (BPW)
B.1.1 General
B.1.2 Composition
B.1.3 Preparation
B.1.4 Performance testing
B.2 Violet red bile glucose (VRBG) agar
B.2.1 Components
B.2.2 Preparation
B.2.3 Preparation of agar plates
B.2.4 Performance testing for the quality assurance of the culture medium
B.3 Non-selective agar medium
B.3.1 General
B.3.2 Composition nutrient agar
B.3.3 Preparation
B.3.4 Preparation of agar plates
B.3.5 Performance testing for the quality assurance of the culture medium
B.4 Glucose OF medium
B.4.1 Composition
B.4.2 Preparation
B.4.3 Performance testing for the quality assurance
B.5 Oxidase reagent
B.5.1 Composition
B.5.2 Preparation
B.5.3 Performance testing for the quality assurance
B.6 Sterile mineral oil
Annex C
Bibliography
Appendix ZZ
ZZ1 General
ZZ2 Variations
Cited references in this standard
Please select a variation to view its description.
| Published | 29/06/2018 |
|---|---|
| Pages | 20 |
Please select a variation to view its pdf.
