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AS 5013.3:2022

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AS 5013.3:2022

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Food microbiology, Method 3: Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of coliforms — Most probable number technique (ISO 4831:2006, MOD)

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Adopts and modifies ISO 4831:2006 for Australia, setting out guidelines for the detection and the enumeration of coliforms. It is applicable to: products intended for human consumption and for the feeding of animals; and environmental samples in the area of food production and food handling.Enumeration is carried out by calculation of the most probable number (MPN) after incubation in a liquid medium at 30 °C or 37°C.

Table of contents
Header
About this publication
Preface
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Detection of coliforms
4.1.1
4.1.2
4.1.3
4.2 Enumeration by the MPN technique
4.2.1
4.2.2
4.2.3
4.2.4
4.2.5
4.2.6
5 Culture media and diluents
5.1 General
5.2 Diluents
5.3 Selective enrichment medium: Lauryl sulfate tryptose broth
5.3.1 Composition
5.3.2 Preparation
5.3.3 Performance testing for the quality assurance of the culture medium
5.4 Confirmation medium: Brilliant green lactose bile broth
5.4.1 Composition
5.4.2 Preparation
5.4.3 Performance testing for the quality assurance of the culture medium
6 Apparatus and glassware
6.1
6.2
6.3
6.4
6.5
6.6
6.7
7 Sampling
8 Preparation of the test sample
9 Procedure (see Annex A)
9.1 Detection method (see Figure A.1)
9.1.1 Test portion and initial suspension
9.1.2 Inoculation and incubation
9.1.2.1
9.1.2.2
9.1.2.3
9.1.3 Confirmation (see Figure A.3)
9.1.3.1
9.1.3.2
9.1.4 Interpretation (see Figure A.1)
9.2 Enumeration method (MPN) (see Figure A.2)
9.2.1 Test portion, initial suspension and dilutions
9.2.2 Inoculation and incubation
9.2.2.1
9.2.2.2
9.2.2.3
9.2.2.4
9.2.2.5
9.2.2.6
9.2.3 Confirmation (see Figure A.3)
9.2.3.1
9.2.3.2
9.2.4 Interpretation (see Figure A.2)
10 Calculation and expression of results
11 Precision
12 Test report
Annex A
Bibliography
Appendix ZZ
ZZ.1 Scope
ZZ.2 Variations

Cited references in this standard
Content history

[Superseded]

[Current]

DR AS 5013.3:2021

Please select a variation to view its description.

Published

04/02/2022
Pages

17

Please select a variation to view its pdf.

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AS 5013.3:2022
$125.14