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AS 5013.26:2020
$125.14
Food microbiology, Method 26: Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157 (ISO 16654:2001/Amd.1:2017, MOD)
This Standard adopts ISO 16654:2001 with modifications for Australia, which specifies a horizontal method for the detection of Escherichia coli serogroup O157.
Table of contents
Header
About this publication
Preface
Foreword
Introduction
1 Scope
2 Normative references
3 Term and definition
4 Principle
5 Culture media, reagents and antisera
5.1
5.1.1
5.1.1.1
5.1.1.2
5.1.2
5.1.2.1
5.1.2.2
5.1.2.3
5.2
5.2.1
5.2.1.1
5.2.1.2
5.2.2
5.2.2.1
5.2.2.2
5.2.3
5.2.3.1
5.2.3.2
5.2.4
5.2.4.1
5.2.4.2
5.3
5.4
5.4.1
5.4.2
5.4.3
5.5
5.5.1
5.5.2
5.6
5.6.1
5.6.2
5.7
5.8
5.8.1
5.8.2
5.9
5.9.1
5.9.2
5.10
6 Apparatus and glassware
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
6.9
6.10
6.11
6.12
6.13
6.14
6.15
6.16
7 Sampling
8 Preparation of test sample
9 Procedure (see annex A)
9.1 Test portion and initial suspension
9.2 Enrichment
9.3 Immunomagnetic separation (IMS)
9.3.1 General
9.3.2 Immunocapture
9.3.3 Separation
9.4 Plating out onto selective agars and identification of E. coli O157 colonies
9.4.1 Plating out
9.4.2 Recognition of typical E. coli O157 colonies
9.5 Confirmation
9.5.1 Selection of colonies
9.5.2 Biochemical confirmation: Indole formation
9.5.3 Serological identification
9.5.3.1 General
9.5.3.2 Elimination of auto-agglutinating isolates
