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AS 5013.26-2009
$80.09
Food microbiology, Method 26: Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157
The objective of this Standard is to specify a horizontal method for the detection of Escherichia coli serogroup O157.
Table of contents
Header
About this publication
Preface
Introduction
1 Scope
2 Normative references
3 Term and definition
4 Principle
5 Culture media, reagents and antisera
5.1
5.1.1 Modified tryptone soya broth (mTSB)
5.1.1.1 Composition
5.1.1.2 Preparation
5.1.2 Novobiocin solution
5.1.2.1 Composition
5.1.2.2 Preparation
5.1.2.3 Preparation of the complete medium
5.2
5.2.1 Base medium
5.2.1.1 Composition
5.2.1.2 Preparation
5.2.2 Potassium tellurite solution
5.2.2.1 Composition
5.2.2.2 Preparation
5.2.3 Cefixime solution
5.2.3.1 Composition
5.2.3.2 Preparation
5.2.4 Complete medium
5.2.4.1 Composition
5.2.4.2 Preparation
5.3
5.4
5.4.1 Composition
5.4.2 Preparation
5.4.3 Preparation of nutrient agar plates
5.5
5.5.1 Composition
5.5.2 Preparation
5.6
5.6.1 Composition
5.6.2 Preparation
5.7
5.8
5.8.1 Composition
5.8.2 Preparation
5.9
5.9.1 Composition
5.9.2 Preparation
5.10
6 Apparatus and glassware
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
6.9
6.10
6.11
6.12
6.13
6.14
6.15
6.16
7 Sampling
8 Preparation of test sample
9 Procedure (see annex A)
9.1 Test portion and initial suspension
9.2 Enrichment
9.3 Immunomagnetic separation (IMS)
9.3.1 General
9.3.2 Immunocapture
9.3.3 Separation
9.4 Plating out onto selective agars and identification of E. coli O157 colonies
9.4.1 Plating out
9.4.2 Recognition of typical E. coli O157 colonies
9.5 Confirmation
9.5.1 Selection of colonies
9.5.2 Biochemical confirmation: Indole formation
9.5.3 Serological identification
9.5.3.1 General
9.5.3.2 Elimination of auto-agglutinating isolates
9.5.3.3 Reaction with E. coli O157 antiserum
9.5.3.4 Positive identification
9.6 Further characterization
10 Quality assurance
10.1 Test strains for quality assurance purposes
10.2 Culture method
11 Expression of results
12 Test report
Annex A
Bibliography
Cited references in this standard
Please select a variation to view its description.
| Published | 18/02/2009 |
|---|---|
| Pages | 13 |
Please select a variation to view its pdf.
