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AS 5013.24.1:2020
$149.01
Food microbiology, Method 24.1: Microbiology of the food chain — Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. — Detection method (ISO 11290-1:2017, MOD)
This Standard adopts ISO 11290 1:2017 with modifications for Australia, which specifies a horizontal method for the detection of L. monocytogenes and Listeria spp. (including L. monocytogenes).This Standard is applicable to —(a) products intended for human consumption and for the feeding of animals; and(b) environmental samples in the area of food production and food handling.
Table of contents
Header
About this publication
Preface
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Primary enrichment in a selective liquid enrichment medium with reduced concentration of selective agents (half-Fraser broth)
4.3 Secondary enrichment with a selective liquid enrichment medium with full concentration of selective agents (Fraser broth)
4.4 Plating out and identification
4.5 Confirmation
5 Culture media and reagents
6 Equipment and consumables
6.1
6.2
6.3
6.4
6.5
6.6
6.7
6.8
6.9
6.10
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion and initial suspension
9.2 Primary enrichment
9.3 Secondary enrichment
9.3.1
9.3.2
9.4 Plating out and identification
9.4.1 General
9.4.1.1
9.4.1.2
9.4.1.3
9.4.1.4
9.4.2 Agar Listeria according to Ottaviani and Agosti
9.4.3 Second selective medium
9.5 Confirmation of Listeria monocytogenes or Listeria spp.
9.5.1 Selection of colonies for confirmation
9.5.1.1
9.5.1.2
9.5.2 Confirmation of L. monocytogenes
9.5.2.1 General
9.5.2.2 Catalase reaction (optional)
9.5.2.3 Motility test (optional)
9.5.2.4 Microscopic aspect (optional in the case of use of agar specific for pathogenic Listeria spp.)
9.5.2.5 Haemolysis tests
9.5.2.5.1 General
9.5.2.5.2 Haemolysis on blood agar
9.5.2.5.3 Haemolysis reaction using red blood corpuscles
