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AS 5013.2-2007
$125.14
Food microbiology, Method 2: Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of Bacillus cereus — Colony-count technique at 30°C (ISO 7932:2004, MOD)
This Standard specifies a horizontal method for the enumeration of viable presumptive Bacillus cereus by means of the colony-count technique at 30 °C. It is applicable to -products intended for human consumption and the feeding of animals, and – environmental samples in the area of food production and food handling.
Table of contents
Header
About this publication
Preface
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1
4.2
4.3
5 Dilution fluid, culture media and reagents
5.1 Dilution fluid
5.2
5.2.1 Base medium
5.2.1.1 Composition
5.2.1.2 Preparation
5.2.2 Polymyxin B solution
5.2.2.1 Composition
5.2.2.2 Preparation
5.2.3 Egg yolk emulsion
5.2.4 Complete medium (MYP agar)
5.2.4.1 Composition
5.2.4.2 Preparation
5.2.5 Preparation of agar plates
5.2.6 Performance testing
5.3 Sheep blood agar
5.3.1 Base medium: Blood agar base No. 2
5.3.1.1 Composition
5.3.1.2 Preparation
5.3.2 Defibrinated sheep blood
5.3.2.1 Complete medium
5.3.2.1.1 Composition
5.3.2.1.2 Preparation
6 Apparatus and glassware
6.1 Apparatus for dry sterilization (oven) or wet sterilization (autoclave)
6.2
6.3
6.4
6.5
6.6
6.7
6.8
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion, initial suspension and dilutions
9.2 Inoculation and incubation
9.2.1
9.2.2
9.2.3
9.2.4
9.3 Counting of the colonies
9.4 Confirmation
9.4.1 Selection and purification of colonies for confirmation
