This Standard sets out a method for estimating the number of colony-forming units (CFUs) in suitable liquids using a membrane filtration technique. The method is applicable only to liquids that can be efficiently filtered without causing a build-up on the filter.
Table of contents
Header
About this publication
Preface
1 Scope
2 Referenced documents
3 Principle
4 Diluents and culture media
5 Apparatus
6 Preparation of apparatus and materials
6.1 Sterilization
6.2 Medium
6.2.1 Liquid medium
6.2.2 Solid medium
7 Filtration procedure
7.1 General
7.2 Procedure
8 Incubation
9 Staining, counting and identification (see Note 1)
