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AS 5013.14.1-2010
$242.18
Food microbiology, Method 14.1: Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations (ISO 7218:2007, MOD)
This Standard applies to the microbiology of food, animal feeding stuffs, the food production environment and the primary production environment.
Table of contents
Header
About this publication
Preface
Introduction
1 Scope
2 Normative references
3 Premises
3.1 General
3.2 Safety considerations
3.3 Laboratory design
3.4 Laboratory areas
3.4.1 General
3.4.2 Areas associated with samples and testing
3.4.3 General areas
3.5 Layout and fittings of the premises
3.5.1 Objectives
3.5.2 Fittings
3.5.3 Other points
3.6 Cleaning and disinfection
4 Staff
4.1 General
4.2 Competence
4.3 Verification of on-going staff competence
4.4 Hygiene
5 Apparatus and equipment
5.1 General
5.2 Protective cabinets
5.2.1 Description
5.2.2 Use
5.2.3 Cleaning and disinfection
5.2.4 Maintenance and inspection
5.3 Balances and gravimetric diluters
5.3.1 Use and measurement uncertainty
5.3.2 Cleaning and disinfection
5.3.3 Performance verification and calibration
5.4 Homogenizers, blenders and mixers
5.4.1 Description
5.4.2 Use
5.4.3 Cleaning and disinfection
5.4.4 Maintenance
5.5 pH meter
5.5.1 Description
5.5.2 Use
5.5.3 Verification and gauging
5.5.4 Maintenance
5.6 Autoclave
5.6.1 Description
5.6.2 Use
5.6.3 Maintenance
5.6.4 Verification and calibration
5.7 Media preparator
5.7.1 Description
5.7.2 Use
5.7.3 Maintenance
5.7.4 Verification
5.8 Incubator
5.8.1 Description
5.8.2 Use
5.8.3 Cleaning and sanitization
5.8.4 Verification
5.9 Refrigerator, cold-storage room
5.9.1 Description
5.9.2 Use
5.9.3 Verification
5.9.4 Maintenance and cleaning
5.10 Freezer and deep freezer
5.10.1 Description
5.10.2 Use
5.10.2.1 Freezer
5.10.2.2 Deep freezer
5.10.3 Verification
5.10.4 Maintenance
5.11 Thermostatically controlled bath
5.11.1 Description
5.11.2 Use
5.11.3 Verification
5.11.4 Maintenance
5.12 Steamers, including boiling-water baths
5.12.1 Description
5.12.2 Use
5.12.3 Maintenance
5.13 Sterilizing oven
5.13.1 Description
5.13.2 Use
5.13.3 Verification
5.13.4 Maintenance
5.14 Microwave oven
5.14.1 Description
5.14.2 Use
5.14.3 Verification
5.14.4 Maintenance
5.15 Glass washer
5.15.1 Description
5.15.2 Use
5.15.3 Verification
5.15.4 Maintenance
5.16 Optical microscope
5.16.1 Description
5.16.2 Use
5.16.3 Maintenance
5.17 Gas burner or wire incinerator
5.17.1 Description
5.17.2 Use
5.17.3 Maintenance
5.18 Dispenser for culture media and reagents
5.18.1 Description
5.18.2 Use
5.18.3 Verification
5.18.4 Cleaning and maintenance
5.19 Vortex mixer
5.19.1 Description
5.19.2 Use
5.19.3 Verification
5.19.4 Maintenance
5.20 Colony-counting device
5.20.1 Description
5.20.2 Use
5.20.3 Verification
5.20.4 Maintenance
5.21 Equipment for culture in a modified atmosphere
5.21.1 Description
5.21.2 Use
5.21.3 Verification
5.21.4 Maintenance
5.22 Centrifuge
5.22.1 Description
5.22.2 Use
5.22.3 Verification
5.22.4 Maintenance
5.23 Hotplate and heating mantle
5.23.1 Description
5.23.2 Use
5.23.3 Maintenance
5.24 Spiral plater
5.24.1 Description
5.24.2 Use
5.24.3 Verification
5.24.4 Maintenance
5.25 Stills, deionizers and reverse-osmosis units
5.25.1 Description
5.25.2 Use
5.25.3 Verification
5.25.4 Maintenance
5.26 Timers and timing devices
5.26.1 Description
5.26.2 Use
5.26.3 Verification
5.26.4 Maintenance
5.27 Pipettes and pipettors
5.27.1 Description
5.27.2 Use
5.27.3 Verification
5.27.4 Maintenance
5.28 Thermometers and temperature-monitoring devices, including automatic recorders
5.28.1 Description
5.28.2 Use
5.28.3 Verification
5.28.4 Maintenance
5.29 Immunomagnetic separator
5.29.1 Description
5.29.2 Use and verification
5.29.3 Maintenance
5.30 Filtration system
5.31 Other equipment and software
6 Preparation of glassware and other laboratory materials
6.1 Preparation
6.2 Sterilization/decontamination
6.2.1 General
6.2.2 Sterilization by dry heat
6.2.3 Sterilization by moist heat (steam)
6.2.4 Decontamination with chemical compounds
6.3 Disposable equipment and materials
6.4 Storage of clean glassware and materials
6.5 Management of sterile glassware and materials
6.6 Use of decontamination and disinfection
6.6.1 Decontamination of disposable equipment
6.6.2 Decontamination of glassware and materials prior to use
6.6.3 Decontamination of glassware and materials after use
6.7 Waste management
6.8 Washing
7 Preparation and sterilization of culture media
8 Laboratory samples
8.1 Sampling
8.1.1 General
8.1.2 Sampling plan
8.2 Transport
8.3 Receipt
8.4 Storage
8.5 Test portion
8.5.1 Specific rules for taking test portions
8.5.2 Conservation and destruction of laboratory samples
9 Examination
9.1 Hygienic precautions during analysis
9.2 Preparation of initial suspension and dilutions
9.2.1 General
9.2.2 Concentration
9.2.2.1 Centrifugation or membrane filtration
9.2.2.2 Immunoseparation
10 Enumeration
10.1 General
10.2 Enumeration using a solid medium
10.2.1 General
10.2.2 Number of Petri dishes per dilution
10.2.3 Pour plate techniques
10.2.3.1 General
10.2.4 Surface inoculation
10.2.4.1 General
10.2.4.2 Spreading-spatula method
10.2.4.3 Spiral-plate method
10.2.4.3.1 General
10.2.4.3.2 Preparation of agar plate
10.2.4.3.3 Plating procedure and counting
10.2.5 Incubation
10.3 Calculation and expression of results obtained with solid media
10.3.1 Counting of colonies
10.3.2 Expression of results
10.3.2.1 General
10.3.2.1.1
10.3.2.1.2
10.3.2.2 Method of calculation: general case (counting of total colonies or typical colonies)
10.3.2.3 Method of calculation: after identification
10.3.2.4 Method of calculation: low counts
10.3.2.4.1 Case when one dish (test sample or initial suspension or first dilution) contains less than 10 colonies
10.3.2.4.2 Case when the dish (test sample or initial suspension or first dilution) contains no colonies
10.3.2.4.3 Special cases
10.3.2.4.3.1 General
10.3.2.4.3.2 Case 1
10.3.2.4.3.3 Case 2
10.3.2.5 Method of calculation: special cases
10.3.2.5.1
10.3.2.5.2
10.3.2.5.3
10.3.2.6 Measurement of uncertainty
10.4 Enumeration of yeasts and moulds
10.4.1 General
10.4.2 Counting of colonies for yeasts and moulds
10.5 Enumeration using a liquid medium
10.5.1 Principle
10.5.2 Inoculation
10.5.2.1 General
10.5.2.2 Procedure
10.5.3 Choice of inoculation system
10.5.3.1 Single-dilution system
10.5.3.2 Multiple-dilution system
10.5.3.3 Symmetric dilution system
10.5.3.4 Non-symmetric dilution system
10.5.4 Incubation
10.5.5 Interpretation of results
10.5.6 Determination of MPN values
10.5.6.1 Mathematical formulae
10.5.6.1.1 Approximate formula for all cases
10.5.6.1.2 “Exact” solution for one series of tubes
10.5.6.1.3 Precision estimates single-dilution assays
10.5.6.1.4 Precision estimates symmetrical multiple-dilution assays
10.5.6.2 MPN tables
10.5.6.2.1 Tables for single-dilution systems
10.5.6.2.2 Tables for multiple-dilution systems: three successive dilutions
10.5.6.3 Computer programs
10.5.7 Expression of results
11 Detection method (qualitative method)
11.1 General
11.2 Principle
11.3 Measurement of uncertainty
12 Confirmation methods
12.1 General
12.2 Preparation of a pure culture
12.3 Gram’s stain (modified Hucker technique)
12.3.1 General
12.3.2 Solutions
12.3.2.1 General
12.3.2.2 Crystal violet solution
12.3.2.2.1 Composition
12.3.2.2.2 Preparation
12.3.2.3 Iodine solution
12.3.2.3.1 Composition
12.3.2.3.2 Preparation
12.3.2.4 Safranin solution
12.3.2.4.1 Composition
12.3.2.4.2 Preparation
12.3.3 Staining technique
12.3.4 Interpretation
12.4 Use of biochemical galleries for identification
12.5 Use of nucleic probes for identification
12.6 Serological methods
12.6.1 General
12.6.2 Slide agglutination tests
12.6.3 Latex agglutination test
13 Test report
14 Validation of microbiological methods
14.1 Validation of reference methods
14.2 Validation of alternative methods
14.3 Validation of in-house methods
15 Quality assurance of results/quality control of performance
15.1 Internal quality control
15.1.1
15.1.2
15.1.3
15.2 Reference strains
15.3 External quality assessment (proficiency testing)
Annex A
Annex B
Bibliography
APPENDIX ZZ
ZZ1 Scope
ZZ2 Variations
Cited references in this standard
Content history
[Superseded]
[Superseded]
AS 5013.14.1-2010 REC:2021
DR 10020
Please select a variation to view its description.
| Published | 03/12/2010 |
|---|---|
| Pages | 70 |
Please select a variation to view its pdf.
