The objective of this Standard is to specify a method, using membrane filtration, for enumerating coliforms, Escherichia coli (E. coli) and thermotolerant coliforms in water other than packaged water.
Table of contents
Header
About this publication
Preface
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and culture media
6 Apparatus
7 Reference cultures
7.1 General
7.2 Positive cultures
7.3 Negative culture
8 Samples
8.1 Laboratory samples
8.2 Dilutions
9 Procedure
9.1 Filtration procedure
9.2 Incubation for combined total coliforms and E. coli enumeration
9.3 Resuscitation and incubation for combined E. coli and thermotolerant coliform enumeration
9.4 Identification of total coliforms and E. coli on MI agar
9.5 Identification of combined total coliforms and E. coli on chromogenic E. coli/coliform selective agar incubated at 36 °C ± 2 °C
9.6 Identification of combined E. coli and thermotolerant coliforms on chromogenic E. coli/coliform selective agar incubated at 44 °C ± 0.5 °C
