Purchase the full subscription package now and enjoy a 40% discount, along with free updates for future editions.
AS 4276.3:2021
$125.14
Water microbiology, Method 3: Enumeration of heterotrophic microorganisms — Pour plate, spread plate, membrane filtration and most probable number techniques
Establishes a standard method for the examination of water, with or without biocides, for the enumeration of heterotrophic microorganisms that covers a choice of possible techniques. KEYWORDS: Water microbiology; Enumeration; Heterotrophic microorganisms
Table of contents
Header
About this publication
Preface
Introduction
1 Scope and general
1.1 Scope
1.2 Normative references
1.3 Terms and definitions
2 Principle
3 Samples
3.1 Laboratory samples
3.2 Dilutions
4 Apparatus
5 Culture media and diluents
6 Quality assurance
6.1 General
6.2 Performance testing of culture media
6.3 Reference cultures
6.3.1 General
6.3.2 Positive culture
7 Techniques
7.1 Pour plate
7.1.1 General
7.1.2 Procedure
7.1.3 Inoculation and pouring of plates
7.1.4 Incubation
7.1.5 Counting of colonies
7.1.6 Calculation of results
7.2 Spread plate
7.2.1 General
7.2.2 Procedure
7.2.3 Incubation
7.2.4 Counting of colonies
7.2.5 Calculation of results
7.3 Spiral plate
7.3.1 General
7.3.2 Procedure
7.3.3 Incubation
7.3.4 Reading of results
7.3.5 Calculation of results
7.4 Membrane filtration
7.4.1 General
7.4.2 Procedure
7.4.3 Incubation
7.4.4 Counting of colonies
7.4.5 Calculation of results
7.5 Most probable number (MPN)
7.5.1 General
7.5.2 Procedure
7.5.3 Reading of results
7.5.4 Calculation of results
8 Test report
Appendix A
Appendix B
B.1 Scope
B.2 Reasoner’s 2A agar (R2A)
B.2.1 Reference
B.2.2 Materials
B.2.3 Apparatus
B.2.4 Procedure
B.3 Plate count agar (PCA)
B.3.1 Reference
B.3.2 Materials
B.3.3 Apparatus
B.3.4 Procedure
B.4 m-HPC agar
B.4.1 Reference
B.4.2 Materials
B.4.3 Apparatus
B.4.4 Procedure
B.5 Yeast extract agar (YEA)
B.5.1 Reference
B.5.2 Materials
B.5.3 Apparatus
B.5.4 Procedure
B.6 Tryptone soy/soya agar (casein soya bean digest agar) (TSA)
