Sets out a method for the preparation of soils, sediments, sludges [including high-particulate-content liquids (slurries)] and biosolids for culture-based bacterial analysis utilizing selective growth media and/or conditions, including most probable number (MPN), presence/absence (PA) and agar plating techniques.
Table of contents
Header
About this publication
Preface
Foreword
1 Scope
2 Referenced documents
3 Definitions
4 Principle
5 Reagents (See Appendix C)
5.1 Soil extraction buffer (SEB)
5.2 Diluents
5.3 1 N and 10 N HCl solution, as required
5.4 1 N NaOH solution
5.5 pH calibration buffers
6 Apparatus
6.1 Apparatus and materials for homogenization
6.1.1 Sterile containers
6.1.2 Sterile container
6.1.3 Sterile wide bore pipettes or equivalent measuring devices
6.1.4 Sterile beakers
6.1.5 Sterilizing equipment
6.1.6 Magnetic stirrer and stir bars (magnetic fleas)
6.1.7 Sterile blender jars or blender homogenizer
6.1.8 Vortex mixer
6.1.9 pH meter
6.1.10 Gloves for handling samples and extraction equipment
6.1.11 Sample handling apparatus
6.1.12 Mechanical shakers
6.1.13 Stomacher (peristaltic blender) and sterile bags
6.1.14 Forceps
6.1.15 80% (v/v) or 70% (w/w) ethanol, or 70% (v/v) or 60% (w/w) isopropanol
6.2 Apparatus for percent solids by drying to constant weight
