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AS 4276.17.1:2016
$87.70
Water microbiology, Method 17.1: Spores of Clostridium perfringens — Membrane filtration method
Sets out a method for enumerating spores of C. perfringens in water, using membrane filtration.
Table of contents
Header
About this publication
Preface
Foreword
1 Scope
2 Referenced documents
3 Definitions
4 Principle
5 Media, reagents and reference cultures
5.1 Culture media (see Appendix B)
5.1.1 Tryptose sulfite cycloserine (TSC) agar
5.1.2 Oleandomycin polymyxin sulphadiazine perfringens (OPSP) agar
5.1.3 Columbia blood agar or other suitable nutrient-rich agar
5.1.4 Columbia blood agar with neomycin (0.01%)
5.1.5 Acid phosphatase reagent
5.2 Reference cultures
5.2.1 Positive culture
5.2.2 Negative culture
5.2.3 Use of reference cultures
6 Apparatus
6.1 Filtration equipment and 0.45 μm membranes
6.2 Anaerobic incubators/jar
6.3 Incubators or water bath
7 Samples
8 Test procedures
9 Confirmation of C. perfringens
9.1 Selection of colonies
9.2 Biochemical confirmation
9.2.1 Acid phosphatase test
9.2.2 Alternative biochemical tests
9.3 Confirmed C. perfringens
10 Calculations
11 Report
12 Bibliography
Appendix A
Appendix B
B1 Scope
B2 Tryptose sulfite cycloserine (TSC) agar
B2.1 TSC base agar
B2.2 TSC selective agent (per 1 L TSC base)
B2.3 Preparation of complete TSC agar
B3 Oleandomycin polymyxin sulphadiazine perfringens (OPSP) agar
B3.1 OPSP base agar
B3.2 OPSP selective agents (per1 L OPSP base)
B3.3 Preparation of complete OPSP agar
B4 Columbia blood agar
B5 Columbia blood agar with neomycin (0.01%)
B6 Acid phosphatase reagent
Cited references in this standard
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| Published | 12/04/2016 |
|---|---|
| Pages | 10 |
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