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AS 4276.14-1995
$47.81
Water microbiology, Method 14: Salmonellae
This Standard sets out a method for the detection of salmonellae in water.
Table of contents
Header
About this publication
PREFACE
1 SCOPE
2 REFERENCED DOCUMENTS
3 CULTURE MEDIA, DILUENTS AND REAGENTS (see AS 4276.2)
3.1 Buffered peptone water
3.2 Rappaport-Vassiliadis (RV) medium
3.3 Mannitol selenite cystine (MSC) broth
3.4 Xylose lysine desoxycholate (XLD) agar
3.5 Bismuth sulfite agar
3.6 Nutrient agar
3.7 Urea agar
3.8 Sucrose peptone water
3.9 Lysine decarboxylase broth and decarboxylase broth base
3.10 ONPG broth
3.11 Formalinized saline solution
3.12 Oxidase reagent (Kovacs’)
4 APPARATUS
4.1 Usual bacteriological glassware
4.2 Apparatus for concentration of microorganisms
4.3 Incubators or water baths
5 REFERENCE CULTURES AND ANTISERA
5.1 Salmonella Salford, 16: l,v: e,n,x, (IMVS culture No. 1710∗)
5.2 Salmonella IIIb, 61: i: z53 (formerly S. arizonae), (IMVS culture No. 1679∗)
5.3 Citrobacter freundii, NCTC 9750 or ATCC 8090
5.4 Use of reference cultures
5.5 Salmonella antisera
5.5.1 Polyvalent ‘O’ (somatic)
5.5.2 Polyvalent ‘H’ specific and non-specific (flagellar)
5.5.3 ‘Vi’ antiserum
6 LABORATORY SAMPLES
7 CONCENTRATION TECHNIQUES
7.1 Moore swab
7.2 Membrane filtration
7.3 Centrifugation
7.4 Diatomaceous earth filtration
8 RESUSCITATION (PRE-ENRICHMENT)
9 SELECTIVE BROTH ENRICHMENT
10 SELECTIVE AGAR CULTURES
11 PURITY CHECK AND OXIDASE TEST
12 BIOCHEMICAL CONFIRMATION
12.1 General
12.2 Commercial identification kits
12.3 Biochemical tests
13 SEROLOGICAL CONFIRMATION
13.1 General
13.2 Pretesting the antisera
13.3 Confirming test cultures
14 INTERPRETATION OF RESULTS
15 FURTHER TESTS
16 COMPLETE IDENTIFICATION
17 REPORT
18 REFERENCES
APPENDIX A
Cited references in this standard
Content history
[Current]
[Available Superseded]
[Superseded]
DR 93222
Please select a variation to view its description.
| Published | 05/03/1995 |
|---|---|
| Pages | 8 |
Please select a variation to view its pdf.
