Specifies a method for isolating and estimating the number of Legionella pneumophila and a range of other Legionella spp. in water. This method is applicable to all water samples, including recreational, industrial, waste and natural waters.
Table of contents
Header
About this publication
Preface
Foreword
1 Scope
2 Referenced documents
3 Principle
4 Safety precautions
5 Culture media, reagents and reference cultures
5.1 Culture media
5.2 Reagents and identification systems
5.2.1 Acid buffer
5.2.2 Speciation/typing system(s)
5.3 Reference cultures
5.3.1 Positive cultures
5.3.2 Use of reference cultures
5.4 Quality control systems
6 Apparatus
7 Sampling, transport and storage
8 Procedure
8.1 General
8.2 Isolation of legionellae
8.2.1 Isolation procedure
8.2.2 Untreated sample
8.2.3 Heat-treated sample
8.2.4 Acid-treated sample
8.3 Incubation
8.4 Examination
8.5 Confirmation of Legionella spp.
8.5.1 General
8.5.2 Procedure
8.5.3 Interpretation
8.6 Legionella speciation and typing
9 Calculations
9.1 Confirmed counts
9.2 Example
10 Test report
Appendix A
Appendix B
B1 Scope
B2 Blood agar (BA)
B3 Buffered charcoal yeast extract agar (BCYE)
B3.1 Formulation
B3.2 Preparation
B3.2.1 Cysteine and iron solutions
B3.2.2 ACES buffer
B3.2.3 Final medium
B3.3 References
B4 Buffered charcoal yeast extract agar with BMPA selective supplement (BMPA)
B4.1 Formulation
B4.2 Preparation
B4.2.1 Cefamandole
B4.2.2 Polymixin B sulfate
B4.2.3 Anisomycin
B4.2.4 Final medium
B4.3 References
B5 Buffered charcoal yeast extract agar with MWY selective supplement (MWY)
B5.1 Formulation
B5.2 Preparation
B5.2.1 Polymyxin B sulfate
B5.2.2 Anisomycin
B5.2.3 Vancomycin hydrochloride
B5.2.4 Bromothymol blue
B5.2.5 Bromocresol purple
B5.2.6 Final medium
B5.3 References
B6 Buffered charcoal yeast extract agar with GVPC selective supplement (GVPC)
B6.1 Formulation
B6.2 Preparation
B6.2.1 Vancomycin hydrochloride
B6.2.2 Polymyxin B sulfate
B6.2.3 Cycloheximide
B6.2.4 Final medium
B6.3 References
B7 Buffered charcoal yeast extract agar with selective supplements (BCYE+AB)
B7.1 Formulation
B7.2 Preparation
B7.2.1 Cefazolin
B7.2.2 Polymyxin B sulfate
B7.2.3 Pimaricin
B7.2.4 Final medium
B7.3 Reference
B8 Buffered charcoal yeast extract medium without L‑cysteine (BCYE-Cys)
