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AS 2252.3-2011
$213.50
Controlled environments, Part 3: Biological safety cabinets Class III — Design
This Standard specifies basic requirements for microbiological safety cabinets with respect to safety and hygiene.
Table of contents
Header
About this publication
PREFACE
Introduction
1 Scope
2 Normative references
3 Definitions
4 Hazards
5 Performance classes
5.1 Leaktightness
5.2 Cleanability
5.3 Sterilizability
5.4 Minimum requirements for performance
6 Classification and verification of performance
6.1 General
6.2 Leaktightness
6.2.1 General
6.2.2 Leaktightness for air
6.2.2.1 Leakage of carcass (class I and class II)
6.2.2.2 Leakage of carcass (class III)
6.2.3 Leaktightness for liquid spillage
6.2.4 Testing for leaktightness aspects and microbiological contamination
6.3 Cleanability
6.4 Sterilizability
7 Safety requirements
7.1 Sealing of openings
7.2 Alarm indicators
7.3 Environmental safety
7.4 MSCs exhaust and anti blowback system
7.5 Filter assemblies for supply and exhaust air
7.6 Volumetric airflow rate and ventilation ratio (class III)
7.7 Cabinet pressure monitoring (class III)
8 Marking and packaging
9 Documentation
Annex A
A.1 Front aperture height
A.2 Lighting
A.3 Sound
A.4 Vibration
A.5 Stability
A.6 Materials, general
A.7 Glass and front windows
A.8 Electrical safety
A.9 Gas supply safety
A.10 Ergonomics
A.11 Temperature
A.12 Glove ports and gloves (class III)
Annex B
B.1 Principle
B.2 Reagents
B.3 Apparatus
B.4 Procedure
B.5 Expression of results
B.6 Test report
Annex C
C.1 General
C.1.1 General principle
C.1.2 Replicate testing
C.2 Microbiological test method
C.2.1 Reagents
C.2.2 Apparatus
C.2.3 Procedure
C.2.4 Calculation
C.2.5 Expression of results
C.2.6 Test report
C.3 Potassium iodide (KI) method
C.3.1 Introduction
C.3.2 Reagents
C.3.3 Apparatus
C.3.3.1
C.3.3.2
C.3.3.3
C.3.3.4
C.3.3.5
C.3.4 Preparation for tests
C.3.5 Procedure
C.3.6 Calculation of the Apf and expression of results
C.3.7 Background tests
C.3.8 Test report
C.4 Nebulizer selection and calibration for microbiological method
C.4.1 Selection criteria
C.4.2 Calibration
C.4.2.1 General
C.4.2.2 Reagents
C.4.2.3 Apparatus
C.4.2.4 Calibration procedure
C.4.2.5 Calculations and expression of results
C.4.2.6 Alternative method for determining the air velocity of the nebulizer
Annex D
D.1 Principle
D.2 Reagents
D.3 Apparatus
D.4 Procedure
D.5 Expression of results
D.6 Test report
Annex E
E.1 Principle
E.2 Reagents
E.3 Apparatus
E.4 Procedure
E.5 Calculation and expression of results
E.6 Test report
Annex F
F.1 Principle
F.2 Reagents
F.3 Apparatus
F.4 Procedure
F.4.1
F.4.2
F.4.3
F.4.4
F.4.5
F.4.6
F.4.7
F.4.8
F.4.9
F.5 Calculation and expression of results
F.6 Test report
Annex G
G.1 Principle
G.2 Apparatus
G.3 Procedure
G.3.1 Class I MSCs
G.3.2 Class II MSCs
G.3.2.1 Downflow
G.3.2.2 Inflow
G.3.3 Class III MSCs
G.3.3.1 Inflow through open glove port
G.3.3.2 Inflow through the inlet filter
G.4 Expression of results
G.5 Test report
Annex H
H.1 General
H.2 Class I MSCs
H.3 Class II MSCs
H.3.1 General
H.3.2 Downflow
H.3.3 Inflow
H.3.4 Stop/start of MSCs
Annex J
J.1 Decontamination and cleaning
J.2 Fumigation
Annex K
K.1 General
K.2 Class I MSCs
K.3 Class II MSCs
K.4 Class III MSCs
Bibliography
Cited references in this standard
Content history
EN 12469:2000
DR AS 2252.3
Please select a variation to view its description.
| Published | 29/06/2011 |
|---|---|
| Pages | 42 |
Please select a variation to view its pdf.
