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AS 5013.14.3-2012
$70.16
Food microbiology, Method 14.3: Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of microorganisms – Colony count at 30°C by the surface plating technique
This Standard specifies a horizontal method for enumeration of microorganisms growing on the surface of a solid medium after aerobic incubation at 30°C.The method is applicable to – products intended for human consumption or for animal feeding stuffs; and – environmental samples in the area of food production and food handling.
Table of contents
Header
About this publication
Preface
1 Scope
2 Referenced documents
3 Definitions
4 Principle
5 Diluents and culture media
5.1 General
5.2 Diluents
5.3 Agar medium: plate count agar (PCA)
5.3.1 Composition
5.3.2 Preparation
5.3.3 Preparation of agar plates
5.3.4 Performance testing
5.3.4.1 General
5.3.4.2 Productivity
6 Apparatus
6.1 General
6.2 Autoclave
6.3 Drying cabinet or incubator
6.4 Incubator
6.5 Water bath
6.6 pH meter
6.7 Petri dishes
6.8 Pipettes
6.9 Colony-counting equipment
6.10 Bottles or flasks, of appropriate capacity
6.11 Spreaders
6.12 Microwave oven or steamer (For melting agar)
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion, initial suspension and dilutions
9.2 Inoculation and incubation
9.3 Enumeration
10 Calculation
11 Test report
Appendix A
A1 General
A2 Principle
A3 Culture media and diluents
A3.1 Sterile distilled water
A3.2 Sodium hypochlorite solution
A4 Apparatus
A4.1 Usual microbiological apparatus
A4.2 Spiral plater
A4.3 Colony counting equipment
A4.4 Disposable sterile 5 mL beakers
A4.5 Prepared agar plates
A5 Sampling
A6 Preparation of test sample
A7 Procedure
A7.1 Test portion and dilutions
A7.2 Setting up
A7.3 Inoculation and incubation
A7.3.1 Inoculation
A7.3.2 Sterility check
A7.3.3 Incubation
A7.4 Enumeration
A7.4.1 Counting grid
A7.4.2 Calibration
A7.4.3 Examination and reporting of spiral plate counts (Manual method)
A7.4.4 Examination, and reporting of spiral plate counts (Using electronic colony counter)
